diff --git a/django/db/models/query.py b/django/db/models/query.py
index 03d12cfa08..1cf2a80994 100644
--- a/django/db/models/query.py
+++ b/django/db/models/query.py
@@ -1331,6 +1331,8 @@ class Prefetch(object):
         self.prefetch_through = lookup
         # `prefetch_to` is the path to the attribute that stores the result.
         self.prefetch_to = lookup
+        if queryset is not None and queryset._iterable_class is not ModelIterable:
+            raise ValueError('Prefetch querysets cannot use values().')
         if to_attr:
             self.prefetch_to = LOOKUP_SEP.join(lookup.split(LOOKUP_SEP)[:-1] + [to_attr])
 
diff --git a/tests/prefetch_related/tests.py b/tests/prefetch_related/tests.py
index ef32320c0a..41eee219b8 100644
--- a/tests/prefetch_related/tests.py
+++ b/tests/prefetch_related/tests.py
@@ -733,6 +733,10 @@ class CustomPrefetchTests(TestCase):
         self.assertEqual(str(warns[0].message), msg)
         self.assertEqual(str(warns[0].message), msg)
 
+    def test_values_queryset(self):
+        with self.assertRaisesMessage(ValueError, 'Prefetch querysets cannot use values().'):
+            Prefetch('houses', House.objects.values('pk'))
+
 
 class DefaultManagerTests(TestCase):