diff --git a/django/contrib/admin/static/admin/css/forms.css b/django/contrib/admin/static/admin/css/forms.css
index abcc0454f1..a778057dc4 100644
--- a/django/contrib/admin/static/admin/css/forms.css
+++ b/django/contrib/admin/static/admin/css/forms.css
@@ -37,16 +37,19 @@ label {
 
 /* RADIO BUTTONS */
 
-form ul.radiolist li {
-    list-style-type: none;
+form div.radiolist div {
+    padding-right: 7px;
 }
 
-form ul.radiolist label {
-    float: none;
-    display: inline;
+form div.radiolist.inline div {
+    display: inline-block;
 }
 
-form ul.radiolist input[type="radio"] {
+form div.radiolist label {
+    width: auto;
+}
+
+form div.radiolist input[type="radio"] {
     margin: -2px 4px 0 0;
     padding: 0;
 }
@@ -106,7 +109,7 @@ form .aligned ul {
     padding-left: 10px;
 }
 
-form .aligned ul.radiolist {
+form .aligned div.radiolist {
     display: inline-block;
     margin: 0;
     padding: 0;
diff --git a/django/contrib/admin/static/admin/css/responsive.css b/django/contrib/admin/static/admin/css/responsive.css
index c7cc3692ed..4c6101f1d9 100644
--- a/django/contrib/admin/static/admin/css/responsive.css
+++ b/django/contrib/admin/static/admin/css/responsive.css
@@ -228,7 +228,7 @@ input[type="submit"], button {
         margin-left: 15px;
     }
 
-    form .aligned ul.radiolist {
+    form .aligned div.radiolist {
         margin-left: 2px;
     }
 
@@ -646,12 +646,13 @@ input[type="submit"], button {
         padding-left: 0;
     }
 
-    form .aligned ul.radiolist {
+    form .aligned div.radiolist {
+        margin-top: 5px;
         margin-right: 15px;
         margin-bottom: -3px;
     }
 
-    form .aligned ul.radiolist:not(.inline) li + li {
+    form .aligned div.radiolist:not(.inline) div + div {
         margin-top: 5px;
     }
 
diff --git a/docs/releases/4.0.2.txt b/docs/releases/4.0.2.txt
index 31f6261b38..8227d98622 100644
--- a/docs/releases/4.0.2.txt
+++ b/docs/releases/4.0.2.txt
@@ -25,3 +25,6 @@ Bugfixes
 * Fixed a regression in Django 4.0 that caused a crash of ``makemigrations`` on
   models without ``Meta.order_with_respect_to`` but with a field named
   ``_order`` (:ticket:`33449`).
+
+* Fixed a regression in Django 4.0 that caused incorrect
+  :attr:`.ModelAdmin.radio_fields` layout in the admin (:ticket:`33407`).