diff --git a/django/utils/tree.py b/django/utils/tree.py
index 2a188acda7..421ad5cd3c 100644
--- a/django/utils/tree.py
+++ b/django/utils/tree.py
@@ -71,7 +71,10 @@ class Node:
         )
 
     def __hash__(self):
-        return hash((self.__class__, self.connector, self.negated) + tuple(self.children))
+        return hash((self.__class__, self.connector, self.negated) + tuple([
+            tuple(child) if isinstance(child, list) else child
+            for child in self.children
+        ]))
 
     def add(self, data, conn_type, squash=True):
         """
diff --git a/docs/releases/2.1.1.txt b/docs/releases/2.1.1.txt
index c83c03b9a5..f6e4bc567f 100644
--- a/docs/releases/2.1.1.txt
+++ b/docs/releases/2.1.1.txt
@@ -18,3 +18,6 @@ Bugfixes
 * Fixed a regression in Django 2.0 where using ``manage.py test --keepdb``
   fails on PostgreSQL if the database exists and the user doesn't have
   permission to create databases (:ticket:`29613`).
+
+* Fixed a regression in Django 2.0 where combining ``Q`` objects with ``__in``
+  lookups and lists crashed (:ticket:`29643`).
diff --git a/tests/utils_tests/test_tree.py b/tests/utils_tests/test_tree.py
index 65f49c06a6..c59398aedb 100644
--- a/tests/utils_tests/test_tree.py
+++ b/tests/utils_tests/test_tree.py
@@ -23,10 +23,12 @@ class NodeTests(unittest.TestCase):
         node3 = Node(self.node1_children, negated=True)
         node4 = Node(self.node1_children, connector='OTHER')
         node5 = Node(self.node1_children)
+        node6 = Node([['a', 1], ['b', 2]])
         self.assertNotEqual(hash(self.node1), hash(self.node2))
         self.assertNotEqual(hash(self.node1), hash(node3))
         self.assertNotEqual(hash(self.node1), hash(node4))
         self.assertEqual(hash(self.node1), hash(node5))
+        self.assertEqual(hash(self.node1), hash(node6))
         self.assertEqual(hash(self.node2), hash(Node()))
 
     def test_len(self):