diff --git a/django/db/models/sql/query.py b/django/db/models/sql/query.py
index b961ac9eeb..8a4f88a9c8 100644
--- a/django/db/models/sql/query.py
+++ b/django/db/models/sql/query.py
@@ -1266,7 +1266,11 @@ class BaseQuery(object):
 
         for alias, aggregate in self.aggregate_select.items():
             if alias == parts[0]:
-                self.having.add((aggregate, lookup_type, value), AND)
+                entry = self.where_class()
+                entry.add((aggregate, lookup_type, value), AND)
+                if negate:
+                    entry.negate()
+                self.having.add(entry, AND)
                 return
 
         opts = self.get_meta()
diff --git a/tests/regressiontests/aggregation_regress/models.py b/tests/regressiontests/aggregation_regress/models.py
index ca2447f505..8a590dca8b 100644
--- a/tests/regressiontests/aggregation_regress/models.py
+++ b/tests/regressiontests/aggregation_regress/models.py
@@ -151,6 +151,19 @@ FieldError: Cannot resolve keyword 'foo' into field. Choices are: authors, id, i
 >>> Book.objects.select_related('publisher').annotate(num_authors=Count('authors')).values()[0]
 {'rating': 4.0, 'isbn': u'013790395', 'name': u'Artificial Intelligence: A Modern Approach', 'pubdate': datetime.date(1995, 1, 15), 'price': Decimal("82.8..."), 'id': 5, 'num_authors': 2, 'publisher_id': 3, 'pages': 1132}
 
+# Regression for #10010: exclude on an aggregate field is correctly negated
+>>> len(Book.objects.annotate(num_authors=Count('authors')))
+6
+>>> len(Book.objects.annotate(num_authors=Count('authors')).filter(num_authors__gt=2))
+1
+>>> len(Book.objects.annotate(num_authors=Count('authors')).exclude(num_authors__gt=2))
+5
+
+>>> len(Book.objects.annotate(num_authors=Count('authors')).filter(num_authors__lt=3).exclude(num_authors__lt=2))
+2
+>>> len(Book.objects.annotate(num_authors=Count('authors')).exclude(num_authors__lt=2).filter(num_authors__lt=3))
+2
+
 """
 }