diff --git a/src/_pytest/assertion/rewrite.py b/src/_pytest/assertion/rewrite.py
index 5fb076d48..456681ab2 100644
--- a/src/_pytest/assertion/rewrite.py
+++ b/src/_pytest/assertion/rewrite.py
@@ -342,7 +342,7 @@ else:
 
         try:
             _write_pyc_fp(fp, source_stat, co)
-            os.rename(proc_pyc, os.fspath(pyc))
+            os.rename(proc_pyc, pyc)
         except OSError as e:
             state.trace(f"error writing pyc file at {pyc}: {e}")
             # we ignore any failure to write the cache file
@@ -356,13 +356,12 @@ else:
 
 def _rewrite_test(fn: Path, config: Config) -> Tuple[os.stat_result, types.CodeType]:
     """Read and rewrite *fn* and return the code object."""
-    fn_ = os.fspath(fn)
-    stat = os.stat(fn_)
-    with open(fn_, "rb") as f:
-        source = f.read()
-    tree = ast.parse(source, filename=fn_)
-    rewrite_asserts(tree, source, fn_, config)
-    co = compile(tree, fn_, "exec", dont_inherit=True)
+    stat = os.stat(fn)
+    source = fn.read_bytes()
+    strfn = str(fn)
+    tree = ast.parse(source, filename=strfn)
+    rewrite_asserts(tree, source, strfn, config)
+    co = compile(tree, strfn, "exec", dont_inherit=True)
     return stat, co
 
 
@@ -374,14 +373,14 @@ def _read_pyc(
     Return rewritten code if successful or None if not.
     """
     try:
-        fp = open(os.fspath(pyc), "rb")
+        fp = open(pyc, "rb")
     except OSError:
         return None
     with fp:
         # https://www.python.org/dev/peps/pep-0552/
         has_flags = sys.version_info >= (3, 7)
         try:
-            stat_result = os.stat(os.fspath(source))
+            stat_result = os.stat(source)
             mtime = int(stat_result.st_mtime)
             size = stat_result.st_size
             data = fp.read(16 if has_flags else 12)
@@ -863,7 +862,7 @@ class AssertionRewriter(ast.NodeVisitor):
                     "assertion is always true, perhaps remove parentheses?"
                 ),
                 category=None,
-                filename=os.fspath(self.module_path),
+                filename=self.module_path,
                 lineno=assert_.lineno,
             )
 
@@ -1105,7 +1104,7 @@ def try_makedirs(cache_dir: Path) -> bool:
     Returns True if successful or if it already exists.
     """
     try:
-        os.makedirs(os.fspath(cache_dir), exist_ok=True)
+        os.makedirs(cache_dir, exist_ok=True)
     except (FileNotFoundError, NotADirectoryError, FileExistsError):
         # One of the path components was not a directory:
         # - we're in a zip file
diff --git a/src/_pytest/pytester.py b/src/_pytest/pytester.py
index 2eada6f46..5aa2fad54 100644
--- a/src/_pytest/pytester.py
+++ b/src/_pytest/pytester.py
@@ -1372,9 +1372,7 @@ class Pytester:
         """
         __tracebackhide__ = True
 
-        cmdargs = tuple(
-            os.fspath(arg) if isinstance(arg, os.PathLike) else arg for arg in cmdargs
-        )
+        cmdargs = tuple(os.fspath(arg) for arg in cmdargs)
         p1 = self.path.joinpath("stdout")
         p2 = self.path.joinpath("stderr")
         print("running:", *cmdargs)
diff --git a/testing/test_assertrewrite.py b/testing/test_assertrewrite.py
index ebe1220bb..9a01fa9ed 100644
--- a/testing/test_assertrewrite.py
+++ b/testing/test_assertrewrite.py
@@ -1681,7 +1681,7 @@ def test_try_makedirs(monkeypatch, tmp_path: Path) -> None:
 
     # monkeypatch to simulate all error situations
     def fake_mkdir(p, exist_ok=False, *, exc):
-        assert isinstance(p, str)
+        assert isinstance(p, Path)
         raise exc
 
     monkeypatch.setattr(os, "makedirs", partial(fake_mkdir, exc=FileNotFoundError()))
diff --git a/testing/test_config.py b/testing/test_config.py
index 1252f31fc..9eddc712d 100644
--- a/testing/test_config.py
+++ b/testing/test_config.py
@@ -595,7 +595,7 @@ class TestConfigAPI:
     def test_getconftest_pathlist(self, pytester: Pytester, tmp_path: Path) -> None:
         somepath = tmp_path.joinpath("x", "y", "z")
         p = tmp_path.joinpath("conftest.py")
-        p.write_text(f"mylist = {['.', os.fspath(somepath)]}")
+        p.write_text(f"mylist = {['.', str(somepath)]}")
         config = pytester.parseconfigure(p)
         assert (
             config._getconftest_pathlist("notexist", path=tmp_path, rootpath=tmp_path)