diff --git a/src/_pytest/fixtures.py b/src/_pytest/fixtures.py
index 43a40a864..481bda8f4 100644
--- a/src/_pytest/fixtures.py
+++ b/src/_pytest/fixtures.py
@@ -372,6 +372,7 @@ def _fill_fixtures_impl(function: "Function") -> None:
         fi = fm.getfixtureinfo(function.parent, function.obj, None)
         function._fixtureinfo = fi
         request = function._request = FixtureRequest(function, _ispytest=True)
+        fm.session._setupstate.prepare(function)
         request._fillfixtures()
         # Prune out funcargs for jstests.
         newfuncargs = {}