diff --git a/changelog/7259.deprecation.rst b/changelog/7259.deprecation.rst
index e450b3599..c0307740d 100644
--- a/changelog/7259.deprecation.rst
+++ b/changelog/7259.deprecation.rst
@@ -1 +1,3 @@
 ``py.path.local`` arguments for hooks have been deprecated. See :ref:`the deprecation note <legacy-path-hooks-deprecated>` for full details.
+
+``py.path.local`` arguments to Node constructors have been deprecated. See :ref:`the deprecation note <node-ctor-fspath-deprecation>` for full details.
diff --git a/doc/en/deprecations.rst b/doc/en/deprecations.rst
index cfe7f489f..b82dd8521 100644
--- a/doc/en/deprecations.rst
+++ b/doc/en/deprecations.rst
@@ -18,6 +18,25 @@ Deprecated Features
 Below is a complete list of all pytest features which are considered deprecated. Using those features will issue
 :class:`PytestWarning` or subclasses, which can be filtered using :ref:`standard warning filters <warnings>`.
 
+.. _node-ctor-fspath-deprecation:
+
+``fspath`` argument for Node constructors replaced with ``pathlib.Path``
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+
+.. deprecated:: 7.0
+
+In order to support the transition from ``py.path.local`` to :mod:`pathlib`,
+the ``fspath`` argument to :class:`~_pytest.nodes.Node` constructors like
+:func:`pytest.Function.from_parent()` and :func:`pytest.Class.from_parent()`
+is now deprecated.
+
+Plugins which construct nodes should pass the ``path`` argument, of type
+:class:`pathlib.Path`, instead of the ``fspath`` argument.
+
+Plugins which implement custom items and collectors are encouraged to replace
+``py.path.local`` ``fspath`` parameters with ``pathlib.Path`` parameters, and
+drop any other usage of the ``py`` library if possible.
+
 
 .. _legacy-path-hooks-deprecated:
 
diff --git a/src/_pytest/deprecated.py b/src/_pytest/deprecated.py
index 0c5db6d53..452128d07 100644
--- a/src/_pytest/deprecated.py
+++ b/src/_pytest/deprecated.py
@@ -101,6 +101,14 @@ HOOK_LEGACY_PATH_ARG = UnformattedWarning(
     "#py-path-local-arguments-for-hooks-replaced-with-pathlib-path",
 )
 
+NODE_CTOR_FSPATH_ARG = UnformattedWarning(
+    PytestDeprecationWarning,
+    "The (fspath: py.path.local) argument to {node_type_name} is deprecated. "
+    "Please use the (path: pathlib.Path) argument instead.\n"
+    "See https://docs.pytest.org/en/latest/deprecations.html"
+    "#fspath-argument-for-node-constructors-replaced-with-pathlib-path",
+)
+
 WARNS_NONE_ARG = PytestDeprecationWarning(
     "Passing None to catch any warning has been deprecated, pass no arguments instead:\n"
     " Replace pytest.warns(None) by simply pytest.warns()."
diff --git a/src/_pytest/nodes.py b/src/_pytest/nodes.py
index 33bf1062b..05cf01fc6 100644
--- a/src/_pytest/nodes.py
+++ b/src/_pytest/nodes.py
@@ -28,6 +28,7 @@ from _pytest.compat import legacy_path
 from _pytest.config import Config
 from _pytest.config import ConftestImportFailure
 from _pytest.deprecated import FSCOLLECTOR_GETHOOKPROXY_ISINITPATH
+from _pytest.deprecated import NODE_CTOR_FSPATH_ARG
 from _pytest.mark.structures import Mark
 from _pytest.mark.structures import MarkDecorator
 from _pytest.mark.structures import NodeKeywords
@@ -101,7 +102,18 @@ def _check_path(path: Path, fspath: LEGACY_PATH) -> None:
         )
 
 
-def _imply_path(path: Optional[Path], fspath: Optional[LEGACY_PATH]) -> Path:
+def _imply_path(
+    node_type: Type["Node"],
+    path: Optional[Path],
+    fspath: Optional[LEGACY_PATH],
+) -> Path:
+    if fspath is not None:
+        warnings.warn(
+            NODE_CTOR_FSPATH_ARG.format(
+                node_type_name=node_type.__name__,
+            ),
+            stacklevel=3,
+        )
     if path is not None:
         if fspath is not None:
             _check_path(path, fspath)
@@ -196,7 +208,7 @@ class Node(metaclass=NodeMeta):
         #: Filesystem path where this node was collected from (can be None).
         if path is None and fspath is None:
             path = getattr(parent, "path", None)
-        self.path = _imply_path(path, fspath=fspath)
+        self.path = _imply_path(type(self), path, fspath=fspath)
 
         # The explicit annotation is to avoid publicly exposing NodeKeywords.
         #: Keywords/markers collected from all scopes.
@@ -573,7 +585,7 @@ class FSCollector(Collector):
                 assert path is None
                 path = path_or_parent
 
-        path = _imply_path(path, fspath=fspath)
+        path = _imply_path(type(self), path, fspath=fspath)
         if name is None:
             name = path.name
             if parent is not None and parent.path != path:
diff --git a/testing/deprecated_test.py b/testing/deprecated_test.py
index bf796a339..0f5e483ce 100644
--- a/testing/deprecated_test.py
+++ b/testing/deprecated_test.py
@@ -215,3 +215,16 @@ def test_deprecation_of_cmdline_preparse(pytester: Pytester) -> None:
             "*Please use pytest_load_initial_conftests hook instead.*",
         ]
     )
+
+
+def test_node_ctor_fspath_argument_is_deprecated(pytester: Pytester) -> None:
+    mod = pytester.getmodulecol("")
+
+    with pytest.warns(
+        pytest.PytestDeprecationWarning,
+        match=re.escape("The (fspath: py.path.local) argument to File is deprecated."),
+    ):
+        pytest.File.from_parent(
+            parent=mod.parent,
+            fspath=legacy_path("bla"),
+        )
diff --git a/testing/plugins_integration/requirements.txt b/testing/plugins_integration/requirements.txt
index 8ef321fd3..89e8b0890 100644
--- a/testing/plugins_integration/requirements.txt
+++ b/testing/plugins_integration/requirements.txt
@@ -4,7 +4,7 @@ pytest-asyncio==0.16.0
 pytest-bdd==4.1.0
 pytest-cov==3.0.0
 pytest-django==4.4.0
-pytest-flakes==4.0.3
+pytest-flakes==4.0.4
 pytest-html==3.1.1
 pytest-mock==3.6.1
 pytest-rerunfailures==10.2