diff --git a/django/db/backends/mysql/features.py b/django/db/backends/mysql/features.py
index 834ea4f88f2..c4706fc1b0d 100644
--- a/django/db/backends/mysql/features.py
+++ b/django/db/backends/mysql/features.py
@@ -336,5 +336,6 @@ class DatabaseFeatures(BaseDatabaseFeatures):
     def supports_expression_indexes(self):
         return (
             not self.connection.mysql_is_mariadb
+            and self._mysql_storage_engine != "MyISAM"
             and self.connection.mysql_version >= (8, 0, 13)
         )