diff --git a/django/contrib/auth/backends.py b/django/contrib/auth/backends.py
index 559d06fe33a..ac86749e533 100644
--- a/django/contrib/auth/backends.py
+++ b/django/contrib/auth/backends.py
@@ -1,10 +1,6 @@
-import inspect
-import warnings
-
 from django.contrib.auth import get_user_model
 from django.contrib.auth.models import Permission
 from django.db.models import Exists, OuterRef, Q
-from django.utils.deprecation import RemovedInDjango31Warning
 
 UserModel = get_user_model()
 
@@ -206,17 +202,7 @@ class RemoteUserBackend(ModelBackend):
                 UserModel.USERNAME_FIELD: username
             })
             if created:
-                args = (request, user)
-                try:
-                    inspect.getcallargs(self.configure_user, request, user)
-                except TypeError:
-                    args = (user,)
-                    warnings.warn(
-                        'Update %s.configure_user() to accept `request` as '
-                        'the first argument.'
-                        % self.__class__.__name__, RemovedInDjango31Warning
-                    )
-                user = self.configure_user(*args)
+                user = self.configure_user(request, user)
         else:
             try:
                 user = UserModel._default_manager.get_by_natural_key(username)
diff --git a/docs/releases/3.1.txt b/docs/releases/3.1.txt
index 3f7e7f261be..5f6af788cea 100644
--- a/docs/releases/3.1.txt
+++ b/docs/releases/3.1.txt
@@ -241,5 +241,8 @@ to remove usage of these features.
 
 * ``django.contrib.staticfiles.storage.CachedStaticFilesStorage`` is removed.
 
+* The ``RemoteUserBackend.configure_user()`` method requires ``request`` as the
+  first positional argument.
+
 * Support for ``SimpleTestCase.allow_database_queries`` and
   ``TransactionTestCase.multi_db`` is removed.
diff --git a/tests/auth_tests/test_remote_user_deprecation.py b/tests/auth_tests/test_remote_user_deprecation.py
deleted file mode 100644
index 1b31d9f0387..00000000000
--- a/tests/auth_tests/test_remote_user_deprecation.py
+++ /dev/null
@@ -1,50 +0,0 @@
-import warnings
-
-from django.contrib.auth.backends import RemoteUserBackend
-from django.contrib.auth.models import User
-from django.test import TestCase, modify_settings, override_settings
-
-
-class CustomRemoteUserBackend(RemoteUserBackend):
-    """Override configure_user() without a request argument."""
-    def configure_user(self, user):
-        user.email = 'user@example.com'
-        user.save()
-        return user
-
-
-@override_settings(ROOT_URLCONF='auth_tests.urls')
-class RemoteUserCustomTest(TestCase):
-    middleware = 'django.contrib.auth.middleware.RemoteUserMiddleware'
-    backend = 'auth_tests.test_remote_user_deprecation.CustomRemoteUserBackend'
-    header = 'REMOTE_USER'
-
-    def setUp(self):
-        self.patched_settings = modify_settings(
-            AUTHENTICATION_BACKENDS={'append': self.backend},
-            MIDDLEWARE={'append': self.middleware},
-        )
-        self.patched_settings.enable()
-
-    def tearDown(self):
-        self.patched_settings.disable()
-
-    def test_configure_user_deprecation_warning(self):
-        """
-        A deprecation warning is shown for RemoteUserBackend that have a
-        configure_user() method without a request parameter.
-        """
-        num_users = User.objects.count()
-        with warnings.catch_warnings(record=True) as warns:
-            warnings.simplefilter('always')
-            response = self.client.get('/remote_user/', **{self.header: 'newuser'})
-            self.assertEqual(response.context['user'].username, 'newuser')
-        self.assertEqual(len(warns), 1)
-        self.assertEqual(
-            str(warns[0].message),
-            'Update CustomRemoteUserBackend.configure_user() to accept '
-            '`request` as the first argument.'
-        )
-        self.assertEqual(User.objects.count(), num_users + 1)
-        user = User.objects.get(username='newuser')
-        self.assertEqual(user.email, 'user@example.com')