diff --git a/src/_pytest/assertion/rewrite.py b/src/_pytest/assertion/rewrite.py
index 0567e8fb8..7627cb968 100644
--- a/src/_pytest/assertion/rewrite.py
+++ b/src/_pytest/assertion/rewrite.py
@@ -33,9 +33,6 @@ PYTEST_TAG = "{}-pytest-{}".format(sys.implementation.cache_tag, version)
 PYC_EXT = ".py" + (__debug__ and "c" or "o")
 PYC_TAIL = "." + PYTEST_TAG + PYC_EXT
 
-AST_IS = ast.Is()
-AST_NONE = ast.NameConstant(None)
-
 
 class AssertionRewritingHook:
     """PEP302/PEP451 import hook which rewrites asserts."""
@@ -857,7 +854,7 @@ class AssertionRewriter(ast.NodeVisitor):
         internally already.
         See issue #3191 for more details.
         """
-        val_is_none = ast.Compare(node, [AST_IS], [AST_NONE])
+        val_is_none = ast.Compare(node, [ast.Is()], [ast.NameConstant(None)])
         send_warning = ast.parse(
             """\
 from _pytest.warning_types import PytestAssertRewriteWarning
diff --git a/testing/test_assertion.py b/testing/test_assertion.py
index 8079c45a0..a8321686d 100644
--- a/testing/test_assertion.py
+++ b/testing/test_assertion.py
@@ -1302,3 +1302,23 @@ def test_exit_from_assertrepr_compare(monkeypatch):
 
     with pytest.raises(outcomes.Exit, match="Quitting debugger"):
         callequal(1, 1)
+
+
+def test_assertion_location_with_coverage(testdir):
+    """This used to report the wrong location when run with coverage (#5754)."""
+    p = testdir.makepyfile(
+        """
+        def test():
+            assert False, 1
+            assert False, 2
+        """
+    )
+    result = testdir.runpytest(str(p))
+    result.stdout.fnmatch_lines(
+        [
+            ">       assert False, 1",
+            "E       AssertionError: 1",
+            "E       assert False",
+            "*= 1 failed in*",
+        ]
+    )