diff --git a/src/_pytest/deprecated.py b/src/_pytest/deprecated.py
index 0d0af725e..274e63b8e 100644
--- a/src/_pytest/deprecated.py
+++ b/src/_pytest/deprecated.py
@@ -37,10 +37,6 @@ YIELD_FIXTURE = PytestDeprecationWarning(
     "Use @pytest.fixture instead; they are the same."
 )
 
-MINUS_K_DASH = PytestRemovedIn7Warning(
-    "The `-k '-expr'` syntax to -k is deprecated.\nUse `-k 'not expr'` instead."
-)
-
 MINUS_K_COLON = PytestRemovedIn7Warning(
     "The `-k 'expr:'` syntax to -k is deprecated.\n"
     "Please open an issue if you use this and want a replacement."
diff --git a/src/_pytest/mark/__init__.py b/src/_pytest/mark/__init__.py
index 7e082f2e6..2eb305109 100644
--- a/src/_pytest/mark/__init__.py
+++ b/src/_pytest/mark/__init__.py
@@ -24,7 +24,6 @@ from _pytest.config import hookimpl
 from _pytest.config import UsageError
 from _pytest.config.argparsing import Parser
 from _pytest.deprecated import MINUS_K_COLON
-from _pytest.deprecated import MINUS_K_DASH
 from _pytest.stash import StashKey
 
 if TYPE_CHECKING:
@@ -189,10 +188,6 @@ def deselect_by_keyword(items: "List[Item]", config: Config) -> None:
     if not keywordexpr:
         return
 
-    if keywordexpr.startswith("-"):
-        # To be removed in pytest 8.0.0.
-        warnings.warn(MINUS_K_DASH, stacklevel=2)
-        keywordexpr = "not " + keywordexpr[1:]
     selectuntil = False
     if keywordexpr[-1:] == ":":
         # To be removed in pytest 8.0.0.
diff --git a/testing/deprecated_test.py b/testing/deprecated_test.py
index e4dcfa67f..18483a4e3 100644
--- a/testing/deprecated_test.py
+++ b/testing/deprecated_test.py
@@ -27,18 +27,6 @@ def test_external_plugins_integrated(pytester: Pytester, plugin) -> None:
         pytester.parseconfig("-p", plugin)
 
 
-def test_minus_k_dash_is_deprecated(pytester: Pytester) -> None:
-    threepass = pytester.makepyfile(
-        test_threepass="""
-        def test_one(): assert 1
-        def test_two(): assert 1
-        def test_three(): assert 1
-    """
-    )
-    result = pytester.runpytest("-k=-test_two", threepass)
-    result.stdout.fnmatch_lines(["*The `-k '-expr'` syntax*deprecated*"])
-
-
 def test_minus_k_colon_is_deprecated(pytester: Pytester) -> None:
     threepass = pytester.makepyfile(
         test_threepass="""