diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index f853528d5..57896c395 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -10,7 +10,7 @@ repos:
     -   id: blacken-docs
         additional_dependencies: [black==20.8b1]
 -   repo: https://github.com/pre-commit/pre-commit-hooks
-    rev: v4.0.1
+    rev: v4.1.0
     hooks:
     -   id: trailing-whitespace
     -   id: end-of-file-fixer
@@ -48,7 +48,7 @@ repos:
     hooks:
     -   id: python-use-type-annotations
 -   repo: https://github.com/pre-commit/mirrors-mypy
-    rev: v0.920
+    rev: v0.930
     hooks:
     -   id: mypy
         files: ^(src/|testing/)
diff --git a/src/_pytest/config/__init__.py b/src/_pytest/config/__init__.py
index 1bdd8effb..dee3d4974 100644
--- a/src/_pytest/config/__init__.py
+++ b/src/_pytest/config/__init__.py
@@ -1429,6 +1429,7 @@ class Config:
             )
         except KeyError:
             return None
+        assert mod.__file__ is not None
         modpath = Path(mod.__file__).parent
         values: List[Path] = []
         for relroot in relroots:
@@ -1574,7 +1575,7 @@ def _strtobool(val: str) -> bool:
 @lru_cache(maxsize=50)
 def parse_warning_filter(
     arg: str, *, escape: bool
-) -> Tuple[str, str, Type[Warning], str, int]:
+) -> Tuple["warnings._ActionKind", str, Type[Warning], str, int]:
     """Parse a warnings filter string.
 
     This is copied from warnings._setoption with the following changes:
@@ -1616,7 +1617,7 @@ def parse_warning_filter(
         parts.append("")
     action_, message, category_, module, lineno_ = (s.strip() for s in parts)
     try:
-        action: str = warnings._getaction(action_)  # type: ignore[attr-defined]
+        action: "warnings._ActionKind" = warnings._getaction(action_)  # type: ignore[attr-defined]
     except warnings._OptionError as e:
         raise UsageError(error_template.format(error=str(e)))
     try:
diff --git a/src/_pytest/monkeypatch.py b/src/_pytest/monkeypatch.py
index 31f95a95a..91d590fb3 100644
--- a/src/_pytest/monkeypatch.py
+++ b/src/_pytest/monkeypatch.py
@@ -55,7 +55,7 @@ def resolve(name: str) -> object:
     parts = name.split(".")
 
     used = parts.pop(0)
-    found = __import__(used)
+    found: object = __import__(used)
     for part in parts:
         used += "." + part
         try:
diff --git a/src/_pytest/pathlib.py b/src/_pytest/pathlib.py
index b23e51d44..def5fa94b 100644
--- a/src/_pytest/pathlib.py
+++ b/src/_pytest/pathlib.py
@@ -539,6 +539,9 @@ def import_path(
     ignore = os.environ.get("PY_IGNORE_IMPORTMISMATCH", "")
     if ignore != "1":
         module_file = mod.__file__
+        if module_file is None:
+            raise ImportPathMismatchError(module_name, module_file, path)
+
         if module_file.endswith((".pyc", ".pyo")):
             module_file = module_file[:-1]
         if module_file.endswith(os.path.sep + "__init__.py"):
diff --git a/src/_pytest/python.py b/src/_pytest/python.py
index 0462665cd..90f035e8f 100644
--- a/src/_pytest/python.py
+++ b/src/_pytest/python.py
@@ -330,6 +330,7 @@ class PyobjMixin(nodes.Node):
         if isinstance(compat_co_firstlineno, int):
             # nose compatibility
             file_path = sys.modules[obj.__module__].__file__
+            assert file_path is not None
             if file_path.endswith(".pyc"):
                 file_path = file_path[:-1]
             path: Union["os.PathLike[str]", str] = file_path
diff --git a/testing/test_conftest.py b/testing/test_conftest.py
index 64c1014a5..92a5ffb72 100644
--- a/testing/test_conftest.py
+++ b/testing/test_conftest.py
@@ -114,6 +114,7 @@ class TestConftestValueAccessGlobal:
             "a", startdir, importmode="prepend", rootpath=Path(basedir)
         )
         assert value == 1.5
+        assert mod.__file__ is not None
         path = Path(mod.__file__)
         assert path.parent == basedir / "adir" / "b"
         assert path.stem == "conftest"
@@ -197,12 +198,14 @@ def test_conftestcutdir(pytester: Pytester) -> None:
     values = conftest._getconftestmodules(
         conf.parent, importmode="prepend", rootpath=pytester.path
     )
+    assert values[0].__file__ is not None
     assert values[0].__file__.startswith(str(conf))
     # and all sub paths get updated properly
     values = conftest._getconftestmodules(
         p, importmode="prepend", rootpath=pytester.path
     )
     assert len(values) == 1
+    assert values[0].__file__ is not None
     assert values[0].__file__.startswith(str(conf))
 
 
@@ -214,6 +217,7 @@ def test_conftestcutdir_inplace_considered(pytester: Pytester) -> None:
         conf.parent, importmode="prepend", rootpath=pytester.path
     )
     assert len(values) == 1
+    assert values[0].__file__ is not None
     assert values[0].__file__.startswith(str(conf))
 
 
diff --git a/testing/test_pathlib.py b/testing/test_pathlib.py
index 24cd71b25..fe5e08f21 100644
--- a/testing/test_pathlib.py
+++ b/testing/test_pathlib.py
@@ -143,6 +143,10 @@ class TestImportPath:
         assert obj.x == 42  # type: ignore[attr-defined]
         assert obj.__name__ == "execfile"
 
+    def test_import_path_missing_file(self, path1: Path) -> None:
+        with pytest.raises(ImportPathMismatchError):
+            import_path(path1 / "sampledir", root=path1)
+
     def test_renamed_dir_creates_mismatch(
         self, tmp_path: Path, monkeypatch: MonkeyPatch
     ) -> None: