diff --git a/CHANGELOG.rst b/CHANGELOG.rst
index 28dd664e1..d36fd5e3c 100644
--- a/CHANGELOG.rst
+++ b/CHANGELOG.rst
@@ -57,11 +57,15 @@
   ``Config.fromdictargs`` now represents its input much more faithfully.
   Thanks to `@bukzor`_ for the complete PR (`#680`_).
 
+* Fix (`#1290`_): support Python 3.5's `@` operator in assertion rewriting.
+  Thanks `@Shinkenjoe`_ for report with test case and `@tomviner`_ for the PR.
+
 
 .. _#1040: https://github.com/pytest-dev/pytest/pull/1040
 .. _#680: https://github.com/pytest-dev/pytest/issues/680
 .. _#1287: https://github.com/pytest-dev/pytest/pull/1287
 .. _#1226: https://github.com/pytest-dev/pytest/pull/1226
+.. _#1290: https://github.com/pytest-dev/pytest/pull/1290
 .. _@MichaelAquilina: https://github.com/MichaelAquilina
 .. _@bukzor: https://github.com/bukzor
 .. _@nicoddemus: https://github.com/nicoddemus
@@ -69,7 +73,8 @@
 .. _@codewarrior0: https://github.com/codewarrior0
 .. _@jaraco: https://github.com/jaraco
 .. _@The-Compiler: https://github.com/The-Compiler
-
+.. _@Shinkenjoe: https://github.com/Shinkenjoe
+.. _@tomviner: https://github.com/tomviner
 
 
 2.8.7
diff --git a/_pytest/assertion/rewrite.py b/_pytest/assertion/rewrite.py
index 9484ba4b3..14b8e49db 100644
--- a/_pytest/assertion/rewrite.py
+++ b/_pytest/assertion/rewrite.py
@@ -453,6 +453,11 @@ binop_map = {
     ast.In: "in",
     ast.NotIn: "not in"
 }
+# Python 3.5+ compatibility
+try:
+    binop_map[ast.MatMult] = "@"
+except AttributeError:
+    pass
 
 # Python 3.4+ compatibility
 if hasattr(ast, "NameConstant"):
diff --git a/testing/test_assertrewrite.py b/testing/test_assertrewrite.py
index d06cb3a00..f43c424ca 100644
--- a/testing/test_assertrewrite.py
+++ b/testing/test_assertrewrite.py
@@ -280,6 +280,19 @@ class TestAssertionRewrite:
             assert False or 4 % 2
         assert getmsg(f) == "assert (False or (4 % 2))"
 
+    @pytest.mark.skipif("sys.version_info < (3,5)")
+    def test_at_operator_issue1290(self, testdir):
+        testdir.makepyfile("""
+            class Matrix:
+                def __init__(self, num):
+                    self.num = num
+                def __matmul__(self, other):
+                    return self.num * other.num
+
+            def test_multmat_operator():
+                assert Matrix(2) @ Matrix(3) == 6""")
+        testdir.runpytest().assert_outcomes(passed=1)
+
     def test_call(self):
         def g(a=42, *args, **kwargs):
             return False