diff --git a/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/ResponseHeaderAssertionConverter.java b/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/ResponseHeaderAssertionConverter.java
index d847ca4251..57508e9425 100644
--- a/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/ResponseHeaderAssertionConverter.java
+++ b/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/ResponseHeaderAssertionConverter.java
@@ -34,7 +34,8 @@ public class ResponseHeaderAssertionConverter extends AssertionConverter<MsRespo
     public boolean isHeaderAssertionValid(MsResponseHeaderAssertion.ResponseHeaderAssertionItem headerAssertionItem) {
         return StringUtils.isNotBlank(headerAssertionItem.getHeader())
                 && StringUtils.isNotBlank(headerAssertionItem.getCondition())
-                && StringUtils.isNotBlank(headerAssertionItem.getExpectedValue());
+                && StringUtils.isNotBlank(headerAssertionItem.getExpectedValue())
+                && BooleanUtils.isTrue(headerAssertionItem.getEnable());
     }
 
     private ResponseAssertion parse2ResponseAssertion(MsResponseHeaderAssertion.ResponseHeaderAssertionItem msAssertion,
diff --git a/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/VariableAssertionConverter.java b/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/VariableAssertionConverter.java
index a50a0391f4..1233ec31d1 100644
--- a/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/VariableAssertionConverter.java
+++ b/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/VariableAssertionConverter.java
@@ -63,7 +63,8 @@ public class VariableAssertionConverter extends AssertionConverter<MsVariableAss
 
     public boolean isValid(MsVariableAssertion.VariableAssertionItem variableAssertionItem) {
         return StringUtils.isNotBlank(variableAssertionItem.getVariableName())
-                && StringUtils.isNotBlank(variableAssertionItem.getCondition());
+                && StringUtils.isNotBlank(variableAssertionItem.getCondition())
+                && BooleanUtils.isTrue(variableAssertionItem.getEnable());
     }
 
     private static String parse2BeanshellJSR233Script(MsVariableAssertion.VariableAssertionItem variableAssertionItem) {
diff --git a/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsJSONPathAssertionItem.java b/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsJSONPathAssertionItem.java
index c5d2dbca16..4090fbe170 100644
--- a/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsJSONPathAssertionItem.java
+++ b/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsJSONPathAssertionItem.java
@@ -3,6 +3,7 @@ package io.metersphere.project.api.assertion.body;
 import io.metersphere.system.valid.EnumValue;
 import jakarta.validation.constraints.NotBlank;
 import lombok.Data;
+import org.apache.commons.lang3.BooleanUtils;
 import org.apache.commons.lang3.StringUtils;
 
 /**
@@ -30,7 +31,7 @@ public class MsJSONPathAssertionItem extends MsBodyAssertionItem {
     private String expectedValue;
 
     public boolean isValid() {
-        return StringUtils.isNotBlank(expression) && StringUtils.isNotBlank(condition);
+        return StringUtils.isNotBlank(expression) && StringUtils.isNotBlank(condition) && BooleanUtils.isTrue(this.getEnable());
     }
 }
 
diff --git a/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsRegexAssertionItem.java b/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsRegexAssertionItem.java
index 5f357a1620..2956a7866e 100644
--- a/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsRegexAssertionItem.java
+++ b/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsRegexAssertionItem.java
@@ -16,6 +16,6 @@ public class MsRegexAssertionItem extends MsBodyAssertionItem {
     private String expression;
 
     public boolean isValid() {
-        return StringUtils.isNotBlank(expression);
+        return StringUtils.isNotBlank(expression) && Boolean.TRUE.equals(this.getEnable());
     }
 }
\ No newline at end of file
diff --git a/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsXPathAssertionItem.java b/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsXPathAssertionItem.java
index 33111a4239..74154cbdee 100644
--- a/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsXPathAssertionItem.java
+++ b/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsXPathAssertionItem.java
@@ -21,6 +21,6 @@ public class MsXPathAssertionItem extends MsBodyAssertionItem {
     private String expectedValue;
 
     public boolean isValid() {
-        return StringUtils.isNotBlank(expression);
+        return StringUtils.isNotBlank(expression) && Boolean.TRUE.equals(this.getEnable());
     }
 }
\ No newline at end of file