diff --git a/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/VariableAssertionConverter.java b/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/VariableAssertionConverter.java
index 1233ec31d1..b4511d7cfd 100644
--- a/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/VariableAssertionConverter.java
+++ b/backend/services/api-test/src/main/java/io/metersphere/api/parser/jmeter/processor/assertion/VariableAssertionConverter.java
@@ -64,7 +64,8 @@ public class VariableAssertionConverter extends AssertionConverter<MsVariableAss
     public boolean isValid(MsVariableAssertion.VariableAssertionItem variableAssertionItem) {
         return StringUtils.isNotBlank(variableAssertionItem.getVariableName())
                 && StringUtils.isNotBlank(variableAssertionItem.getCondition())
-                && BooleanUtils.isTrue(variableAssertionItem.getEnable());
+                && BooleanUtils.isTrue(variableAssertionItem.getEnable())
+                && !StringUtils.equals(variableAssertionItem.getCondition(), MsAssertionCondition.UNCHECK.name());
     }
 
     private static String parse2BeanshellJSR233Script(MsVariableAssertion.VariableAssertionItem variableAssertionItem) {
diff --git a/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsJSONPathAssertionItem.java b/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsJSONPathAssertionItem.java
index 4090fbe170..8f4b560ffd 100644
--- a/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsJSONPathAssertionItem.java
+++ b/backend/services/project-management/src/main/java/io/metersphere/project/api/assertion/body/MsJSONPathAssertionItem.java
@@ -1,5 +1,6 @@
 package io.metersphere.project.api.assertion.body;
 
+import io.metersphere.sdk.constants.MsAssertionCondition;
 import io.metersphere.system.valid.EnumValue;
 import jakarta.validation.constraints.NotBlank;
 import lombok.Data;
@@ -31,7 +32,10 @@ public class MsJSONPathAssertionItem extends MsBodyAssertionItem {
     private String expectedValue;
 
     public boolean isValid() {
-        return StringUtils.isNotBlank(expression) && StringUtils.isNotBlank(condition) && BooleanUtils.isTrue(this.getEnable());
+        return StringUtils.isNotBlank(expression)
+                && StringUtils.isNotBlank(condition)
+                && BooleanUtils.isTrue(this.getEnable()
+                && !StringUtils.equals(condition, MsAssertionCondition.UNCHECK.name()));
     }
 }