diff --git a/django/contrib/auth/tests/test_views.py b/django/contrib/auth/tests/test_views.py
index fa8b38f4c5..3dd8953d88 100644
--- a/django/contrib/auth/tests/test_views.py
+++ b/django/contrib/auth/tests/test_views.py
@@ -475,8 +475,7 @@ class LoginTest(AuthViewsTestCase):
             self.assertEqual(response.context['site_name'], site.name)
         else:
             self.assertIsInstance(response.context['site'], RequestSite)
-        self.assertTrue(isinstance(response.context['form'], AuthenticationForm),
-                     'Login form is not an AuthenticationForm')
+        self.assertIsInstance(response.context['form'], AuthenticationForm)
 
     def test_security_check(self, password='password'):
         login_url = reverse('login')
diff --git a/django/contrib/formtools/tests/wizard/test_forms.py b/django/contrib/formtools/tests/wizard/test_forms.py
index fedb799669..9ae99cc89b 100644
--- a/django/contrib/formtools/tests/wizard/test_forms.py
+++ b/django/contrib/formtools/tests/wizard/test_forms.py
@@ -246,11 +246,11 @@ class SessionFormTests(TestCase):
     def test_init(self):
         request = get_request()
         testform = SessionWizardView.as_view([('start', Step1)])
-        self.assertTrue(isinstance(testform(request), TemplateResponse))
+        self.assertIsInstance(testform(request), TemplateResponse)
 
 
 class CookieFormTests(TestCase):
     def test_init(self):
         request = get_request()
         testform = CookieWizardView.as_view([('start', Step1)])
-        self.assertTrue(isinstance(testform(request), TemplateResponse))
+        self.assertIsInstance(testform(request), TemplateResponse)
diff --git a/django/contrib/gis/geoip/tests.py b/django/contrib/gis/geoip/tests.py
index 9631a192ab..06558e0093 100644
--- a/django/contrib/gis/geoip/tests.py
+++ b/django/contrib/gis/geoip/tests.py
@@ -106,7 +106,7 @@ class GeoIPTest(unittest.TestCase):
             self.assertEqual('TX', d['region'])
             self.assertEqual(713, d['area_code'])
             geom = g.geos(query)
-            self.assertTrue(isinstance(geom, GEOSGeometry))
+            self.assertIsInstance(geom, GEOSGeometry)
             lon, lat = (-95.4010, 29.7079)
             lat_lon = g.lat_lon(query)
             lat_lon = (lat_lon[1], lat_lon[0])
diff --git a/django/contrib/gis/tests/geoapp/tests.py b/django/contrib/gis/tests/geoapp/tests.py
index 3501fb6a7f..2e572c4052 100644
--- a/django/contrib/gis/tests/geoapp/tests.py
+++ b/django/contrib/gis/tests/geoapp/tests.py
@@ -201,7 +201,7 @@ class GeoModelTest(TestCase):
         as_text = 'ST_AsText' if postgis else 'asText'
         cities2 = City.objects.raw('select id, name, %s(point) from geoapp_city' % as_text)
         self.assertEqual(len(cities1), len(list(cities2)))
-        self.assertTrue(isinstance(cities2[0].point, Point))
+        self.assertIsInstance(cities2[0].point, Point)
 
 
 @skipUnless(HAS_GEOS and HAS_SPATIAL_DB, "Geos and spatial db are required.")
diff --git a/django/contrib/gis/tests/relatedapp/tests.py b/django/contrib/gis/tests/relatedapp/tests.py
index 67059120a7..6f0c568803 100644
--- a/django/contrib/gis/tests/relatedapp/tests.py
+++ b/django/contrib/gis/tests/relatedapp/tests.py
@@ -179,8 +179,8 @@ class RelatedGeoModelTest(TestCase):
         for m, d, t in zip(gqs, gvqs, gvlqs):
             # The values should be Geometry objects and not raw strings returned
             # by the spatial database.
-            self.assertTrue(isinstance(d['point'], Geometry))
-            self.assertTrue(isinstance(t[1], Geometry))
+            self.assertIsInstance(d['point'], Geometry)
+            self.assertIsInstance(t[1], Geometry)
             self.assertEqual(m.point, d['point'])
             self.assertEqual(m.point, t[1])
 
@@ -255,7 +255,7 @@ class RelatedGeoModelTest(TestCase):
         qs = Location.objects.filter(id=5).annotate(num_cities=Count('city')).values('id', 'point', 'num_cities')
         self.assertEqual(1, len(qs))
         self.assertEqual(2, qs[0]['num_cities'])
-        self.assertTrue(isinstance(qs[0]['point'], GEOSGeometry))
+        self.assertIsInstance(qs[0]['point'], GEOSGeometry)
 
     # TODO: The phantom model does appear on Oracle.
     @no_oracle
diff --git a/django/contrib/gis/tests/test_measure.py b/django/contrib/gis/tests/test_measure.py
index 643a1b1794..94284fb28a 100644
--- a/django/contrib/gis/tests/test_measure.py
+++ b/django/contrib/gis/tests/test_measure.py
@@ -93,7 +93,7 @@ class DistanceTest(unittest.TestCase):
         self.assertEqual(d5, 50)
 
         a5 = d1 * D(m=10)
-        self.assertTrue(isinstance(a5, Area))
+        self.assertIsInstance(a5, Area)
         self.assertEqual(a5.sq_m, 100 * 10)
 
         with self.assertRaises(TypeError):
diff --git a/django/contrib/sites/tests.py b/django/contrib/sites/tests.py
index 8f80087c5b..fadd4aa81d 100644
--- a/django/contrib/sites/tests.py
+++ b/django/contrib/sites/tests.py
@@ -26,7 +26,7 @@ class SitesFrameworkTests(TestCase):
     def test_site_manager(self):
         # Make sure that get_current() does not return a deleted Site object.
         s = Site.objects.get_current()
-        self.assertTrue(isinstance(s, Site))
+        self.assertIsInstance(s, Site)
         s.delete()
         self.assertRaises(ObjectDoesNotExist, Site.objects.get_current)
 
@@ -57,7 +57,7 @@ class SitesFrameworkTests(TestCase):
             "SERVER_PORT": "80",
         }
         site = get_current_site(request)
-        self.assertTrue(isinstance(site, Site))
+        self.assertIsInstance(site, Site)
         self.assertEqual(site.id, settings.SITE_ID)
 
         # Test that an exception is raised if the sites framework is installed
@@ -68,7 +68,7 @@ class SitesFrameworkTests(TestCase):
         # A RequestSite is returned if the sites framework is not installed
         with self.modify_settings(INSTALLED_APPS={'remove': 'django.contrib.sites'}):
             site = get_current_site(request)
-            self.assertTrue(isinstance(site, RequestSite))
+            self.assertIsInstance(site, RequestSite)
             self.assertEqual(site.name, "example.com")
 
     def test_domain_name_with_whitespaces(self):
diff --git a/django/test/testcases.py b/django/test/testcases.py
index 354c4d3963..95d0d8e399 100644
--- a/django/test/testcases.py
+++ b/django/test/testcases.py
@@ -620,8 +620,8 @@ class SimpleTestCase(unittest.TestCase):
         # test that max_length and min_length are always accepted
         if issubclass(fieldclass, CharField):
             field_kwargs.update({'min_length': 2, 'max_length': 20})
-            self.assertTrue(isinstance(fieldclass(*field_args, **field_kwargs),
-                                       fieldclass))
+            self.assertIsInstance(fieldclass(*field_args, **field_kwargs),
+                                  fieldclass)
 
     def assertHTMLEqual(self, html1, html2, msg=None):
         """
diff --git a/tests/backends/tests.py b/tests/backends/tests.py
index c8c1875e26..ee1e56676f 100644
--- a/tests/backends/tests.py
+++ b/tests/backends/tests.py
@@ -646,7 +646,7 @@ class BackendTestCase(TestCase):
         Test that cursors can be used as a context manager
         """
         with connection.cursor() as cursor:
-            self.assertTrue(isinstance(cursor, CursorWrapper))
+            self.assertIsInstance(cursor, CursorWrapper)
         # Both InterfaceError and ProgrammingError seem to be used when
         # accessing closed cursor (psycopg2 has InterfaceError, rest seem
         # to use ProgrammingError).
@@ -661,7 +661,7 @@ class BackendTestCase(TestCase):
         # psycopg2 offers us a way to check that by closed attribute.
         # So, run only on psycopg2 for that reason.
         with connection.cursor() as cursor:
-            self.assertTrue(isinstance(cursor, CursorWrapper))
+            self.assertIsInstance(cursor, CursorWrapper)
         self.assertTrue(cursor.closed)
 
 
diff --git a/tests/forms_tests/tests/test_forms.py b/tests/forms_tests/tests/test_forms.py
index d2e80d5b4d..2db40ef55c 100644
--- a/tests/forms_tests/tests/test_forms.py
+++ b/tests/forms_tests/tests/test_forms.py
@@ -1908,7 +1908,7 @@ class FormsTestCase(TestCase):
 
         field = ChoicesField()
         field2 = copy.deepcopy(field)
-        self.assertTrue(isinstance(field2, ChoicesField))
+        self.assertIsInstance(field2, ChoicesField)
         self.assertFalse(id(field2.fields) == id(field.fields))
         self.assertFalse(id(field2.fields[0].choices) ==
                          id(field.fields[0].choices))
@@ -2152,7 +2152,7 @@ class FormsTestCase(TestCase):
         e.append('Foo')
         e.append(ValidationError('Foo%(bar)s', code='foobar', params={'bar': 'bar'}))
 
-        self.assertTrue(isinstance(e, list))
+        self.assertIsInstance(e, list)
         self.assertIn('Foo', e)
         self.assertIn('Foo', forms.ValidationError(e))
 
diff --git a/tests/model_fields/tests.py b/tests/model_fields/tests.py
index 754ad20e5d..e8c798adc5 100644
--- a/tests/model_fields/tests.py
+++ b/tests/model_fields/tests.py
@@ -298,7 +298,7 @@ class BooleanFieldTests(unittest.TestCase):
         # conversions are applied with an offset
         b5 = BooleanModel.objects.all().extra(
             select={'string_col': 'string'})[0]
-        self.assertFalse(isinstance(b5.pk, bool))
+        self.assertNotIsInstance(b5.pk, bool)
 
     def test_select_related(self):
         """